Sign in →

Test ID: TPNI Troponin I, Serum

Reporting Name

Troponin I, S

Useful For

Exclusion diagnosis of acute myocardial infarction

Specimen Type


Specimen Required

Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Ambient  24 hours
  Refrigerated  24 hours

Reference Values

≤0.04 ng/mL

Reference values have not been established for patients <17 years of age.

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.- 4 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
TPNI Troponin I, S 89579-7


Result ID Test Result Name Result LOINC Value
TPNI Troponin I, S 89579-7

Clinical Information

Troponin is a complex that regulates the contraction of striated muscle. It consists of 3 subunits (C, T, and I) that are located periodically along the thin filament of the myofibrils. Troponin I inhibits actomyosin ATPase.


Troponin I is an inhibitory protein and exhibits in 3 isoforms: cardiac muscle, slow-twitch skeletal muscle, and fast-twitch skeletal muscle. The cardiac form of troponin I has 31 amino acid residues on its N-terminal, not present in the skeletal forms, which allow for specific polyclonal and monoclonal antibody development. The cardiac specificity of this isoform improves the accuracy of diagnosis in patients with acute or chronic skeletal muscle injury and possible concomitant myocardial injury.


Troponin I is the only troponin isotope present in the myocardium and is not expressed during any developmental stage in skeletal muscle. Troponin I is released into the bloodstream within hours of the onset of symptoms of myocardial infarction or ischemic damage. It can be detected at 3 to 6 hours following onset of chest pain with peak concentrations at 12 to 16 hours, and remains elevated for 5 to 9 days.


There are, on occasions, elevations of cardiac troponin T (cTnT) which we use clinically which can be due to skeletal muscle disease. One way to unmask such elevations is to measure cardiac troponin I (cTnI), which will be normal in that circumstance. In addition, at times there are interferences that can cause spurious increases or decreases in cTnT values. Conceptually, these same interferences can occur with cTnI but in any given case, the likelihood of having both assays be confounded in that way is highly unusual. Thus, potential false-positives would be unmasked by a normal cTnI and false-negatives by an elevated value.


A reference range study was conducted using the ADVIA Centaur TnI-Ultra assay based on guidance from the Clinical and Laboratory Standards Institute (CLSI) Protocol C28-A2.25. The study, which used 1,845 fresh serum, lithium heparin plasma, and EDTA plasma samples from 648 apparently healthy individuals ranging from 17 to 91 years of age, demonstrated a 99th percentile of 0.04 ng/mL (mcg/L).(1)

Clinical Reference

1. Package insert: Siemens Centaur XP, TnI, 04744371 Rev H, 2008-09

2. Apple F: Acute myocardial infarction and coronary reperfusion: serum cardiac markers for the 1990s. Am J Clin Pathol 1992;97:217-226

3. Adams JE 3rd, Bodor GS, Davila-Romain VG, et al: Cardiac troponin I: A marker for cardiac injury. Circulation 1993;88:101-106

4. Corin SJ, Jushasz O, Zhu L, et al: Structure and expression of the human slow twitch skeletal muscle troponin I gene. J Biol Chem 1994;269:10651-10659

Analytic Time

Same day/1 day

Method Name

Chemiluminometric Immunoassay


If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen. 

Mayo Clinic Laboratories | Cardiology Catalog Additional Information: